Böcker i Methods in Visualization-serien

In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy.

Offers researchers with a reference that can show them how to equip their labs with the right materials and methods. Taking care to explain the history behind the techniques and to demonstrate their use, this book presents the theory, principles, and protocols supplemented by hundreds of illustrations.

Covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two fresh approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope levels. This title provides theoretical consideration, as well as practical approaches to in situ PCR.

Radioligand binding in situ is the most sensitive method for detecting available receptors. Based on the authors' experience in the field, this title details these procedures and provides step-by-step methods for visualizing receptors via radioligand binding. It covers various practical procedures, including radioligand and tissue preparations.