Böcker i Neuromethods-serien

This volume looks at the framework in which different scientific disciplines are integrated into the latest studies in behavioral neurogenetics, and covers new approaches toward understanding the system that controls behavior across the animal kingdom. automated behavior analysis using a YOLO-based object detection system;

Recent developments in lesion-symptom mapping (LSM) have spurred rapid growth. The chapters cover the definition and types of brain lesions, how to prepare them for analysis, standard LSM methods, network-based LSM methods, and approaches of transient lesions induced by brain stimulation.

This volume discusses the latest experimental and clinical approaches to study seizures and epilepsy. Chapters in this book cover detailed protocols for ex vivo and in vitro experimental models and widely-used in vivo (acute and chronic seizure) models in epilepsy research.

This volume explores the recent advances in the study of translational paths in central inflammation and focuses on ongoing pathophysiological processes and the transition between inflammatory stages and progressive states with neurodegeneration. Chapters cover topics such as pathophysiological hallmarks of neuroinflammation from tissue damage to reorganization; connecting studies of mouse models; and investigations of humans with multiple sclerosis. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and practical, Translational Methods for Multiple Sclerosis Research is a valuable resource for researchers who want to learn more about this chronic ad progressive disease, and pave the way for new advancements.

This volume highlights various techniques used to study multiple aspects of mGlu receptor function. Chapters in this book cover topics such as modern approaches for the development and screening of allosteric modulators; fluctuations in intracellular calcium induced by mGlu receptor activation; dopamine-glutamate interactions in the central nervous system; methods for assessing mGlu receptor localization; and common techniques for evaluating mGlu modulation in behavior in rodents, including stress and alcohol drinking. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and practical, Metabotropic Glutamate Receptor Technologies provides researchers with a starting point to address important questions regarding mGlu and its importance. Researchers working in the fields of neuroscience and physiology of multiple peripheral systems will find this volume most useful.

This volume covers the latest methods used in clinical neurochemistry laboratories for both clinical practice and research. Chapters in this book discuss topics such as techniques for cerebrospinal fluid (CSF) collection, pre-analytical processing, and basic CSF analysis; an examination of biomarkers including ELISA and automated immunochemical assays for amyloid and tau markers for Alzheimer¿s disease; the analysis of neurofilaments by digital ELISA; and an example of successful novel immunoassay development. In the Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and thorough, Cerebrospinal Fluid Biomarkers is a valuable resource for clinicians and researchers to use in CSF labs and CSF courses.

This volume explores the latest models and techniques used to study brain tumor biology. Chapters in this book are organized into four sections: in vivo models, ex vivo models, treatments in mice, and clinical imaging.

Electrophysiological methods are used at nearly all levels of biological research today. Dr. Yan Dong, an expert in utilizing electrophysiological techniques for brain research with fifteen years of experience, and Peter Neumann hope this work can act as a concise and practical aid to remedy the current situation.

This volume focuses on the optogenetics workflow, and covers topics on viral vectors, targeting strategies, choice on opsins, animal models and readouts, and applications in systems neuroscience.

This volume focuses on protein analysis covering a wide spectrum of the utility of mass spectrometry within neurobiological disciplines. The chapters in this book discuss label (iTRAQ, TMT, protein arrays) and label-free workflows (SWATH, MALDI Imaging, and label-free quantitation). Other chapters look at experimental strategies targeted to the identification and quantitation of specific lipids and post-translational modifications, as well as proteomic workflows that focus on characterization of subcellular proteomes. The last few chapters in the book describe various bioinformatics pipelines used to analyze the molecular data derived from high-throughput transcriptomic and proteomic experiments on brain tissue. The Neuromethods series offers chapters with key advice and procedure specifics to empower the readers to successfully achieve their own scientific and experimental goals.Thorough and comprehensive, Current Proteomic Approaches Applied to Brain Function is a valuable resource for graduate students and postdoctoral fellows interested in neuroproteomics, as well as researchers looking for further insight into the growing field of mass spectrometry in neuroscience.

This volume describes a broad spectrum of experimental approaches for investigating structure, function, and transport of neuronal mitochondria in health and disease. Most of these approaches were only recently developed and range from electron tomography-based 3D reconstruction of mitochondrial cristae to patch clamp recording from mitochondria in intact neurons. The chapters in this book cover topics such as mitochondrial proteomics, fluorescence lifetime imaging, respirometry and mitophagy, as well as optical approaches based on the use of genetically engineered fluorescent sensors for monitoring synaptic ATP and axonal ROS generation, mitochondrial Ca2+ cycling and pH changes, and mitochondrial dynamics and axonal trafficking in live neurons. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Cutting-edge and comprehensive, Techniques to Investigate Mitochondrial Function in Neurons is a valuable and useful resource for a broad range of investigators interested in the function of neuronal mitochondria in health and disease states.

There are detailed methods on leading techniques for stem cell genetic engineering and intra-organ transplantation for research and therapeutic purposes, in vivo imaging of donor stem cells, and essential requirements for setting up a new stem cell laboratory.

This volume provides properties, biological function, methods,and approaches for manipulating nAChRs in different organisms. NicotinicAcetylcholine Receptor Technologies guides readers through moleculartechniques and behavioral tests used to investigate nicotinic drugs,chronoamperometry, emerging technologies and methods for the analysis ofnAChRs, single nucleotide polymorphism (SNP), fluorescence techniques, spectralconfocal, allosteric modulators of a7-nAChRs, and a comprehensive evolutionalrelation for most nAChR subunits, in both vertebrate and invertebrate species.Written for the popular Neuromethods series, chapters include thekind of detail and key implementation advice that ensures successful results inthe laboratory. Authoritative and practical, Nicotinic AcetylcholineReceptor Technologies aims to ensure successful results in thefurther study of this vital field.

This second edition provides updated and expanded chapters that critically address the issues or rodent stroke modeling, from choosing the model and outcome measures, designing the experiment, conducting and analyzing it, to reporting it in a scientific publication.

Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory. Authoritative and practical, Neurotransmitter Transporters: Investigative Methods aims to ensure successful results in thefurther study of this vital field.

Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.Authoritative and practical, Experimental Neurosurgery in Animal Models aims to ensure successful results in the further study of this vital field.

This volume is directed at individuals interested in the field of neuroscience who are novices or experts in the use of transmission electron microscopy. The goal of is to provide a comprehensive series of chapters on how this tool can be used to study the brain with a strong emphasis on successful hands¿on application of the electron microscopy technique. Written in the popular Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Concise and easy-to-use, Transmission Electron Microscopy Methods for Understanding the Brain aims to ensure successful results in the further study of this vital field.

This volume highlights proteomics studies of quantitative PTM changes in both peripheral and central nervous system proteomes utilizing the most recent advances in mass spectrometry. Chapters include practical information pertaining to the fundamentals of sample preparation, liquid chromatography, and tandem mass spectrometry instrumental analysis and will elucidate best practices in the interpretation of data using modern bioinformatics approaches. Written for the popular Neuromethods series, chapters include the kind of detail and key implementation advice that ensures successful results in the laboratory.Authoritative and practical, Analysis of Post-Translational Modifications and Proteolysis in Neuroscience aims to ensure successful results in the further study of this vital field.

This volume explores current viewpoints and knowledge gaps in the field of traumatic brain injury (TBI). The chapters in this book cover topics ranging from development of in vitro and animal TBI models, to diagnostic imaging and disease monitoring in patients. Designing pre-clinical and clinical trials is also discussed. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Practical and thorough, Pre-Clinical and Clinical Methods in Brain Trauma Research is a valuable resource for both scientists and clinical researchers interested in learning about important techniques and their applications in the field of TBI.

This volume explores both simple and sophisticated techniques used in the study of different types of lateralization of brain and behavior. Part II covers neurobiological methods used to reveal lateralization, such as lesion studies, electrophysiology and pharmacology, early gene expression, and new optogenetic methods.

Chapters focus on in-vivo recordings, voltage-gated channel recording and analysis, dendritic and axonal recordings, synaptic current recording and analysis, advanced fluorescent techniques, optogenetics and voltage-sensitive dye imaging, and finally channel and neuronal modeling.

Discovering hidden recurring patterns in observable behavioral processes is an important issue frequently faced by numerous advanced students and researchers across many research areas, including psychology, biology, sports, robotics, media, finance, and medicine. As generally, themany powerful methods included in statistical software packages were not developed for this kind of analysis, discovering such patterns has proven a particularly difficult task, due to a lack of a) adequate formalized models of the kinds of patterns to look for, b) corresponding detection algorithms and c) their implementation in available software. The research described in this book is based on the application of such pattern types, algorithms and software developed from the late seventies to the present in the context of research in collaboration with human and animal behavioral research teams at internationally leading universities in the US and Europe, thus testing the usefulness and validity of the pattern types, algorithms and software in numerous research areas. With the (scale independent statistical hierarchical and fractal-like) T-Pattern at its heart, a set of proposed pattern types, called the T-System, forms the basis for the search algorithms implemented as the software THEME (TM) (vs. 6), which is easily available in free educational and full commercial versions.

This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.

This volume discusses techniques to synthesize and functionalize nanoparticles, monitor their delivery and uptake, and identify and evaluate their lethal and non-lethal effects on the metabolic activity of the nervous system.

This volume discusses the latest techniques and methodologies used in the neurotrophin field to study the physiology of the Brain-Derived Neurotrophic Factor (BDNF).

This volume details methods for the analyses of specific lipid classes and lipidomics analyses of cells such as lymphocytes and oocytes.