Böcker i Neuromethods-serien

The growth of the field of eating disorder research has led to a vast array of empirical articles, and the development of new animal models that can be used to study these disorders continues to stimulate new research.

With an ever-increasing elderly population and the resultant rising levels of dementia-related disorders, preclinical research based on animal models is pivotal to our knowledge of underlying molecular mechanisms and drug discovery aiming at the development of therapeutic strategies alleviating or preventing the neurological devastation.

As the presence of genetically modified animal models in research laboratories has multiplied, the role of genetic factors in the pathogenesis of brain disorders has become particularly important.

Playing an important role in the treatment of neurological disorders, the delivery of drugs to central nervous system (CNS), both administered directly and administered systematically for targeted action, encounters a major challenge in the form of the blood-brain barrier (BBB), which limits the access of drugs to the brain substance.

Preclinical research related to mood and anxiety disorders relies extensively upon mouse behavioral tests and models, the use of which continues to increase as a greater number of underlying susceptibility genes are discovered, new targets for medications are identified, and clinical studies reveal novel neurobiological risk factors.

This volume describes different computational methods encompassing ligand-based approaches (QSAR, pharmcophore), structure-based approaches (homology modeling, docking, molecular dynamics simulation), and combined approaches (virtual screening) with applications in anti-Alzheimer drug design.

This volume presents the latest methods and approaches used to better characterize the properties of the structural human brain, and also explores various dysfunctional and non-dysfunctional neuroanatomical variations. This book is divided into 3 parts: methods, non-clinical applications, and clinical applications.

Providing a spectrum of models that is reflective of the various species that can be utilized in experimentation on disorders across a broad range of developmental disabilities, this volume collects expert contributions involved in investigation of the causes, outcomes, treatment, and prevention.

This volume provides current methods to analyze the properties of designer receptors exclusively activated by designer drugs (DREADDs) in vitro and to measure the biological responses of DREADD activation in different neuronal populations in vivo.

Applying neurophysiological methods to the study of brain-behavior relationships proved to be a major advance in the early days of neuroscience research.

Societal, ethical, and cost-related issues, not to mention the need for sound scientific methods, have led to new and refined methods for the evaluation of health risks associated with neurotoxic compounds, relevant and predictive of exposure, relatively inexpensive, and ideally amenable to high throughput analysis and a reduction in animal use.

This volume discusses the latest techniques and methodologies used in the neurotrophin field to study the physiology of the Brain-Derived Neurotrophic Factor (BDNF).

The second edition of this volume provides up-to-date methods on the main methodological aspects of functional MRI (fMRI), applying fMRI to the study of central nervous system, and future evolutions of fMRI techniques.

This volume presents classical approaches to in vivo neuropharmacology and neurophysiology, such as c-fos, electrochemistry, microdialysis microstimulation, and push-up superfusion. It also explores exciting new methods for behavioral analysis, and techniques based on optogenetics and non-invasive magnetic resonance imaging. The chapters of this book cover topics such as principles of stereotaxy, pharmaco-based fMRI and neurophysiology in humans and non-human primates, electrical nerve stimulation and central microstimulation, involvement of neurotransmitters in mnemonic processes, the impact of cannabinoids on motor activity, as well as the involvement of nitric oxide in neurotoxicity produced by psychostimulant drugs. Each chapter also discusses difficulties, tips, tricks, and precautions to take. Neuromethods series style chapters include the kind of detail and key advice from the specialists needed to get successful results in your own laboratory.Cutting-edge and practical, In Vivo Neuropharmacology and Neurophysiology is a valuable resource for experienced and less experienced investigators of brain function and brain disorders.

The chapters in this book discuss the use of fluorescent metal probes, synchrotron-based X-ray microscopy, ICP-MS, laser ablation-ICP-MS, laser-based tissue microdissection, MRI image analysis, fractionation of cell tissue samples for metal analysis, and metal treatment of cells.

This volume discusses the requirements, advantages, and limitations of studying cell culture. The chapters in this book cover topics such as in vitro blood-brain barrier functional assays in human iPSC-based models; neuron-glia interactions examines with in vitro co-culture; epigenetic changes in cultures neurons and astrocytes; rat brain slices; brain punching technique; and using microRNA for in vitro neurotoxicity testing and related disorders. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory. Authoritative and cutting-edge, Cell Culture Techniques, Second Edition is a valuable resource for students and experiences researchers who are interested in learning more and making risk decisions in this evolving field.

This volume discusses techniques to synthesize and functionalize nanoparticles, monitor their delivery and uptake, and identify and evaluate their lethal and non-lethal effects on the metabolic activity of the nervous system.

This volume provides comprehensive information and detailed laboratory protocols used in the study of prion diseases. Chapters also provide readers with detailed information about kuru, the clinical description of prion diseases, and the detection of prion protein and biomarkers.

This volume presents methods for the analysis of genomic variability in vertebrate neurons and broadens our knowledge in the ways we understand the brain and its neurons. The chapters in this book are divided into 5 parts, and cover the following topics: principles and approaches for discovery of somatic mosaicism in the brain, aneuploidy and ploidy variation, DNA copy number variation, LINE-1 retrotransposition, and genetic and genomic mosaicism in aging and disease. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and authoritative, Genomic Mosaicism in Neurons and Other Cell Types is a valuable resource for learning about the latest techniques for the analysis of genome and genetic mosaicism in vertebrate neurons.

This volume presents techniques and recently developments in extracellular recording. The chapters in this book explore topics such as mouse, rat and zebrafish models; extracellular recordings on human cognition; single-unit recordings and multi-electrode arrays; and recordings in perinatal and early developing mice. In Neuromethods series style, chapters include the kind of detail and key advice from the specialists needed to get successful results in your laboratory.Cutting-edge and authoritative, Extracellular Recording Approaches is a valuable resource that provides readers with up-to-date applications and methods to study brain regions involved in sensory, motor, and cognitive functions.