Covers methods of in situ polymerase chain reaction (PCR) and reverse transcription PCR (RT-PCR), two fresh approaches in visualizing very low amounts of DNA and mRNA in tissues and cell cultures at the light and electron microscope levels. This title provides theoretical consideration, as well as practical approaches to in situ PCR.
In Situ hybridization allows the visualization of specific DNA/RNA sequences in individual cells in tissue sections, single cells, or chromosome preparations, and is an especially important method for studying DNA and RNA in heterogeneous cell populations. This book delves into in situ hybridization methods through the use of light microscopy.
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