Böcker av Nicole Stone

This detailed volume features techniques to explore the complex interface that separates the systemic circulation from the central nervous system, known as the blood-brain barrier (BBB). Beginning with an introduction to its physiology, the book continues with sections on using pluripotent stem cells in models of the BBB, co-culture, permeability and transwell models, microfluidic and chip models, as well as models to study specific BBB pathologies. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, The Blood-Brain Barrier: Methods and Protocols collects a wide range of methodologies which will aid all researchers in the fascinating world of the blood-brain barrier.

It has been nearly a year since Serena Rae has learned she is a Witch. She has accepted this truth, but it has not been easy. The secrets and lies have taken their toll. Only her boyfriend and soulmate, Damian Drake, truly understands. Being a Vampire extraordinaire helps. After a great summer, Serena and Damian prepare for their next chapter, but before they can leave, the impossible happens. Now Serena must embark on a rescue mission to save Damian, and herself, from an enemy in the shadows. Serena will have to come to terms with who she is and what she can do. She will need every ounce of strength she has.Is she powerful enough?