Böcker av Rajib Deb

This detailed book explores new molecular biological techniques for the detection of both antigens and antibodies of porcine diseases. Assays examined within this collection include improved methods of nucleic acid extraction, polymerase chain reaction (PCR), droplet digital PCR (ddPCR), polymerase spiral reaction (PSR), cross-priming amplification (CPA), enzyme-linked immunosorbent assay (ELISA), as well as peptide nucleic acid (PNA) based tools, aptamer-based tools, and lateral flow assays and different immune assay-based diagnostics for porcine diseases. As part of the Springer Protocols Handbooks series, the present book contains the type of in-depth knowledge on important diagnostics tools that provide a vital source of information for scientists, researchers, and students from different fields. Authoritative and practical, Protocols for the Diagnosis of Pig Viral Diseases aims to guide researchers toward developing new technologies for the diagnosis of pig pathogens.

Rapeseed mustard cake (RMC) can be readily used as a protein supplement in lactating cows diet due to its availability and being cheaper than other oil cakes. The use of RMC as protein supplement is constrained due to its higher level of glucosinolates that have variable adverse effect on palatability, intake, nutrient utilization, thyroid hormone status and overall performance of the animals. The new improved varieties of rapeseed mustard cultivar which is low in both erucic acid (

Paratuberculosis (Johne's disease) is a chronic infectious, granulomatous enteric disease of domestic and wild ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map). To date, no effective prophylactic candidates are available for early protection against the paratuberculosis infection in animals. Numerous secretory proteins Map have shown to be potent immunogens and can be used as subunit vaccines/DNA vaccine and also can be used as a specific detection .This book, therefore provides a novel antigen family protein of Map, PPE (proline-proline-glutamic acid rich) an unique antigen, which has been characterized, expressed in prokaryotic as well as eukaryotic system to preliminary study it's existence and further immunoreactivity was studied alone as well as with an adjuvant model gamma interferon in murine model to elucidate its importance to develop DNA vasccine against paratuberculosis infection in animals.

Foot-and-mouth disease (FMD) is one of the highly contagious diseases of domestic animals which has highly economic importance. The disease has got transboundary importance and rank first in list A of infectious diseases of animals, as per World Organization for Animal Health, Paris (OIE). Effective control of this disease needs sensitive, specific and quick diagnostic tools at each tier of control strategy. In this book we have described the various immune-molecular tools for the specific detection of the virus from the clinical materials.