Böcker av Roger L. Lundblad

This brilliant work describes the application of protein modification to proteomic research, covering sample preparation, sample prefractionation, analytical technologies, and validation issues in depth. Written by a recognized expert, it merges current proteomics concepts into established solution protein chemistry concepts. The author gives parti

Examining the chemical modification of biological polymers and the emerging applications of this technology, Chemical Modification of Biological Polymers reflects the change in emphasis in this subsection of biotechnology from the study of protein structure and function toward applications in therapeutics and diagnostics. HighlightsThe basic organic chemistry of the modification proteins, nucleic acids, oligosaccharides, polysaccharides, and their applicationsNew analytical technologies used to characterize the chemical modification of biological polymersIdentification of in vivo, non-enzymatic chemical modification of biological polymersSpecific chemical modifications to generate biopharmaceutical products This book covers the basics on the organic chemistry underlying the chemical modification of biopolymers, including updates on the use of various chemical reagents. It describes the current status of chemical modification of biological polymers and emerging applications of this technology in biotechnology. These technologies are important for the manufacture of conjugate proteins used in drug delivery, for the preparation of nucleic acid microarrays, and for the preparation of hydrogels and other materials used in tissue engineering.

Conformational analysis is becoming more critical in the development of generic biopharmaceuticals. This book describes methods used to establish comparability between potentially identical products, providing researchers with a critical description of studies drawn from the current literature. The text covers physical methods, such as differential scanning calorimetry, light scanning, and analytical ultracentrifugation, as well as chemical methods, including hydrogen¿deuterium exchange and trace labeling. The author shows how these methods support comparability claims for generic biologicals and assure structural integrity of active pharmaceutical ingredients in final drug products.

A comprehensive assessment of the use of biomarkers in diagnostics and prognostics, this book covers the history and current application of biomarkers, explores the technology used for the validation and study of biomarkers, and compares the various technologies used to identify and measure biomarkers. It investigates the evolution of the underlying technology and discusses the translation of basic science to clinical laboratory use and potential commercial applications. He also covers proteomics and proteomic technologies and their applications in the identification of biomarkers.

A comprehensive review of reagents used for the chemical modification of proteins, the fourth edition represents a major revision. The completely updated text is substantially larger and includes five new chapters: Alkylating Agents, Acylating Agents, Nitration and Nitrosylation, Oxidation, and Modification of Proteins with Reducing Agents. The format has been markedly revised, presenting information based on the chemical nature of the modifying material and on the amino acid residue modified. Much of the information is in tabular form to enable the rapid location of cited material.

A comprehensive assessment of the use of biomarkers in diagnostics and prognostics, this book covers the history and current application of biomarkers, explores the technology used for the validation and study of biomarkers, and compares the various technologies used to identify and measure biomarkers. It investigates the evolution of the underlying technology and discusses the translation of basic science to clinical laboratory use and potential commercial applications. He also covers proteomics and proteomic technologies and their applications in the identification of biomarkers.

Conformational analysis is becoming more critical in the development of generic biopharmaceuticals. This book covers physical methods, such as differential scanning calorimetry, light scanning, and analytical ultracentrifugation, as well as chemical methods, including hydrogen-deuterium exchange and trace labeling.

Includes chapters on the use of classical protein chemistry in food, the protein and carbohydrate chemistry of adhesives, glues and sealants, protein chemistry and hydrogels, and the use of chemical modification to prepare protein therapeutics. This book also demonstrates use and the development of biotech applications.