Böcker av Tarso B. Ledur Kist

Fluorescent Dye Labels and StainsThe only comprehensive database of fluorophores and their physical and photochemical propertiesFluorophores are chemical compounds that strongly absorb in the ultraviolet, visible, and/or near-infrared and with bright emission in these ranges. As a result, they are exceptionally valuable as dyes for various analytical processes, capable of labelling and staining particular targets for purposes of fluorescent imaging, sensitive detection, and quantification (exhibiting linear responses over very wide concentration ranges). These compounds are many and varied, and panoramic views of their options, physical properties and their reactions to light excitations can be critical to their successful integration into chemical analysis, pharmaceutical analysis, clinical analysis, microscopies, optical bioimaging, cancer imaging, real-time PCR, flow cytometry, multiplexing in proteomics, life sciences in general, and many other high-tech fields (material sciences, traceability, photovoltaics, quantum computing).Fluorescent Dye Labels and Stains incorporates a comprehensive database of such substances and their characteristics. It provides an introduction to basic theories and foundational terminology, in addition to both the molecular structures and photophysical properties of an enormous range of fluorophores. Assembled over the course of a distinguished career in biochemistry, this database presents valuable information that has never before been available in a single volume.Readers will also find:* Molecular and photochemical information of over 700 fluorophores* A database of parameters, including light excitation ranges, molar absorption coefficients, fluorescence quantum yields, molecular brightness, and many more* Information derived from multiple disciplines, including microscopy, nanoscopy, biochemistry, and molecular biologyFluorescent Dye Labels and Stains is the essential reference for pharmaceutical and biomedical researchers and professionals, academics who study molecular biology or organic chemistry, and any professional whose work includes strong and photostable molecular absorptions and fluorescence.

Presents the theory and applications of Toroidal Capillary, Microchip, and Slab Electrophoresis to analytical chemists across a range of disciplinesWritten by one of the developers of Toroidal Capillary Electrophoresis (TCE), this book is the first to present this novel analytical technique, in detail, to the field of analytical chemistry.The exact expressions of separation efficiency, resolution, peak capacity, and many other performance indicators of the open and toroidal layouts are presented and compared.Featuring numerous illustrations throughout, Open and Toroidal Electrophoresis: Ultra-High Separation Efficiencies in Capillaries, Microchips and Slabs offers chapters covering: Solvents and Buffer Solutions; Fundamentals of Electrophoresis; Open Layout; and Toroidal Layout. Confronting Performance Indicators is next, followed by chapters on High Voltage Modules and Distributors; Heat Removal and Temperature Control; and Detectors. The book finishes with an examination of the applications of Toroidal Electrophoresis.The first book to offer a detailed account of Toroidal Electrophoresis--written by one of its creators* Compares the toroidal layouts with the well-established open layouts of the three most used platforms (Capillary, Microchip, and Slab)* Provides solutions to many of the experimental issues arising in electromigration techniques and discusses the voltage distributors and detectors that are compatible with the toroidal layouts* Richly illustrated with a large number of useful equations showing the relationships between important operational parameters and the performance indicatorsOpen and Toroidal Electrophoresis is aimed at method developers and separation scientists working in clinical analysis, and food analysis, as well as those in pharmacology, disease biomarker applications, and nucleic acid analysis using the Capillary, Microchip, or slab Platform. It will also benefit undergraduate and graduate students of inorganic analytical chemistry, organic analytical chemistry, bioanalysis, pharmaceutical sciences, clinical sciences, and food analysis.