Böcker utgivna av Humana Press Inc.

Playing an important role in the treatment of neurological disorders, the delivery of drugs to central nervous system (CNS), both administered directly and administered systematically for targeted action, encounters a major challenge in the form of the blood-brain barrier (BBB), which limits the access of drugs to the brain substance.

Vertebrate Embryogenesis: Embryological, Cellular and Genetic Methods attempts to address the increasingly important need of straddling species boundaries in the context of a single research program by compiling research protocols used in a wide range of vertebrate species.

Driven by methodological success in identifying reliable lineage markers, regulatory T cells have quickly been recognized as the most numerous subset of immune regulators in the body with critical functions in a wide array of immune responses.

Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required.

Chemical library technologies have brought about dramatic changes in the drug discovery process, and, though still evolving, they have become an integral part of ongoing drug discovery research.

This volume covers an array of topics from the employment of the chick CAM model using fluorescent proteins and other fluorescent probes, to intravital fluorescent imaging, as well as 3-dimensional imaging, and design instructions on how to create new and improved far-red and infrared fluorescent proteins, to name a few.

Chapters outline methods to study miRNA functions in various cell types from a single cell type level to entire model organisms, and present studies of miRNAs in the context of viruses and the immune response.

Recent efforts to characterize genetic variation in the human genome, coupled with the rapidly developing field of genomics, have lead directly to the development of new and innovative approaches to the identification of genes contributing to complex human diseases.

This book fills the gaps in our understanding of the identification of triggers, recognition of clinical presentations, understanding of the natural history of these reactions, and selection of treatment strategies including those focused on cellular and molecular targets.

This changing de?nition of disease means that today's undergraduate or graduate student in dentistry (and its related ?elds) must be in a position not only to acquire new knowledge in the future but also to be able to evaluate the information and apply it in a clinically relevant setting.

As the presence of genetically modified animal models in research laboratories has multiplied, the role of genetic factors in the pathogenesis of brain disorders has become particularly important.

The kingdom Fungi constitutes an independent group equal in rank to that of plants and animals. Fungi are highly amenable to molecular work, and a few fungal species serve as model systems to study basic processes with results that are applicable to many organisms, including humans.

Interest in the field of microbial toxins is ever growing and spreading across a broad spectrum of scientific disciplines. In an effort to supplement the available reference texts on toxins, Microbial Toxins: Methods and Protocols includes protocols on mold fungus toxins, with some focus on aflatoxins.

From the early days when RNA interference was a strange artifact in worms to the 2006 Noble Prize received by Fire and Mello and the current clinical trials, the field of RNA interference has grown at a breakneck pace.

Membrane proteins play a key role in numerous pathologies such as cancer, cystic fibrosis, epilepsy, hyperinsulinism, and Alzheimer's disease, yet studies on these and other disorders are hampered by a lack of information about the proteins involved.

While there is a wide selection of 'by experts, for experts' books in statistics and molecular biology, there is a distinct need for a book that presents the basic principles of proper statistical analyses and progresses to more advanced statistical methods in response to rapidly developing technologies and methodologies in the field of molecular biology.  Statistical Methods in Molecular Biology  strives to fill that gap by covering basic and intermediate statistics that are useful for classical molecular biology settings and advanced statistical techniques that can be used to help solve problems commonly encountered in modern molecular biology studies, such as supervised and unsupervised learning, hidden Markov models, methods for manipulation and analysis of high-throughput microarray and proteomic data, and methods for the synthesis of the available evidences. This detailed volume offers molecular biologists a book in a progressive style where basic statistical methods are introduced and gradually elevated to an intermediate level, while providing statisticians knowledge of various biological data generated from the field of molecular biology, the types of questions of interest to molecular biologists, and the state-of-the-art statistical approaches to analyzing the data.  As a volume in the highly successful Methods in Molecular Biology(TM) series, this work provides the kind of meticulous descriptions and implementation advice for diverse topics that are crucial for getting optimal results.   Comprehensive but convenient, Statistical Methods in Molecular Biology will aid students, scientists, and researchers along the pathway from beginning strategies to a deeper understanding of these vital systems of data analysis and interpretation within one concise volume.  "Here is a comprehensive book that systematically covers both basic and advanced statistical topics in molecularbiology, including parametric and nonparametric, and frequentist and Bayesian methods.  I am highly impressed by the breadth and depth of the applications. I strongly recommend this book for both statisticians and biologists who need to communicate with each other in this exciting field of research."- Robert C. Elston, PhD., Director, Division of Genetic and Molecular Epidemiology, Case Western Reserve University "An extraordinary exposition of the central topics of modern molecular biology, presented by practicing experts who weave together rigorous theory with practical techniques and illustrative examples."- George C. Newman, MD, PhD, Chairman, Neurosensory Sciences, Albert Einstein Medical Center "I cannot think of anything we need now in translation research field more than more efficient cross talk between molecular biology and statistics. This book is just on target. It fills the gap."- Iman Osman, MB, BCh, MD, Director, Interdisciplinary Melanoma Cooperative Program, New York University Langone Medical Center

While commercial instruments have expanded the usage and the related literature has increased, the quality of surface plasmon resonance (SPR) research has been hindered by a lack of knowledge of the processes that influence the SPR signal.  In Surface Plasmon Resonance: Methods and Protocols , experts in the field present a wide variety of applications involving commercially available SPR instruments. The heart of the SPR technique is to construct a dedicated surface for an assay. Protocols describe such surfaces for many needs, including the study of membrane bound proteins. Beyond the protocols-based chapters, the volume also highlights the backgrounds of vital issues in the use of SPR, including processes occurring within the hydrogel environment of sensors and on lipid membrane surfaces as well as the analysis of kinetic information.  Written in the highly successful Methods in Molecular Biology (TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Surface Plasmon Resonance: Methods and Protocols fills a need for well-described, hands-on SPR experimental protocols and promises to inspire the adaptation of these techniques to fit the needs of labs around the world.