DNA Vaccines

Part I: Vector design For vaccination
1. A Novel Cre Recombinase Mediated In Vivo Minicircle (CRIM) DNA Vaccine Platform for Veterinary Application
Yanlong Jiang, Guilian Yang, and Chunfeng Wang

2. Vaccination with Messenger RNA - a Promising Alternative to DNA Vaccination
István Tombácz, Drew Weissman, and Norbert Pardi

3. Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency
Kenneth Lundstrom

Part II: DNA Vaccine Adjuvants and Immunostimulatory Response
4. CpG Oligonucleotides as Vaccine Adjuvants
Neslihan Kayraklioglu, Begum Horuluoglu, and Dennis M. Klinman

5. Molecular Adjuvants for DNA Vaccines: Application, Design, Preparation and Formulation
Ailar Sabbaghi and Amir Ghaemi

6. Assessing Antigen-specific Cellular Immune Responses Upon HIV/SIV Plasmid DNA Vaccination in the Non-human Primate Model
Xintao Hu, Barbara K. Felber, and Antonio Valentin

Part III Biotechnological Processes to Obtain DNA Vaccines
7. Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design
Luís A. Passarinha

8. Primary Purification of Plasmid DNA Using Differential Isopropanol Precipitation
Alexandra Wagner, A. Rita Silva-Santos, Sara Sousa Rosa, Sophie Gierak, Ana M. Azevedo, and Duarte Miguel F. Prazeres

9. Scale-Up of Plasmid DNA Downstream Process Based on Chromatographic Monoliths
Urh Černigoj and Aleš Štrancar

10. Purification of Plasmid DNA by Multimodal Chromatography
A. Rita Silva-Santos, Sara Sousa Rosa, Duarte Miguel F. Prazeres, and Ana M. Azevedo

11. Minicircle DNA Vaccine Purification and E7 Antigen Expression Assessment
Ana M. Almeida, Dalinda Eusébio, João A. Queiroz, Fani Sousa, and Ângela Sousa

Part IV: DNA Vaccine Delivery
12. Tumor-Specific CD8+ T Cell Responses Induced by DNA Vaccination
Pablo Cáceres-Morgado and Alvaro Lladser

13. Therapeutic DNA Vaccine Against HPV16-Associated Cancer
Meihua Yu and Janin Chandra

14. Bulk and Microfluidic Synthesis of Stealth and Cationic-Liposomes for Gene Delivery Applications
Lucimara Gaziola de la Torre, Amanda da Costa e Silva de Noronha Pessoa, Bruna Gregatti de Carvalho, Thiago Bezerra Taketa, Ismail Es, and Gabriel Perli

15. Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines Field
Diana Costa, Ângela Sousa, Rúben Faria, Ana Raquel Neves, and João A. Queiroz

16. Main Features of DNA Based Vectors for Use in Lactic Acid Bacteria and Update Protocols
Nina D. Coelho-Rocha, Fernanda A. L. Barroso, Laísa M. Tavares, Ester S. S. dos Santos, Vasco Azevedo, Mariana M. Drumond, and Pamela Mancha-Agresti

Part V: DNA Vaccine Transfer to Clinical Trials
17. Ethics of DNA Vaccines Transfer for Clinical Research
Ana Cristina Ramalhinho and Miguel Castelo-Branco

18. Preparation of an Academic Clinical TrialPreparation of an academic clinical trial
Ana Cristina Ramalhinho and Miguel Castelo-Branco