Böcker utgivna av Springer-Verlag New York Inc.

This book addresses the major periodontal pathogens implicated as causal agents in periodontal disease, including Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Prevotella spp.

This book addresses the major periodontal pathogens implicated as causal agents in periodontal disease, including Porphyromonas gingivalis, Tannerella forsythia, Treponema denticola, Fusobacterium nucleatum, Aggregatibacter actinomycetemcomitans, and Prevotella spp.

Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.

Authoritative and practical, Polypeptide Materials: Methods and Protocols serves to reflect the inter-disciplinary nature of molecular biology as well as the importance of developing innovative measurement methods to advance this vital area of research.

This detailed book aims to provide readers with critical information to accomplish the synthesis of nanosystems for the purpose of supramolecular entities complexing with drugs, targeted drug delivery system characterization, as well as the study of the physical-chemical interactions that govern the stability and properties of these systems.

This detailed book aims to provide readers with critical information to accomplish the synthesis of nanosystems for the purpose of supramolecular entities complexing with drugs, targeted drug delivery system characterization, as well as the study of the physical-chemical interactions that govern the stability and properties of these systems.

This detailed new edition aims to provide a range of methods and protocols for studying vascular morphogenesis in vivo and in vitro to reflect up-to-date advances in the field.

This detailed new edition aims to provide a range of methods and protocols for studying vascular morphogenesis in vivo and in vitro to reflect up-to-date advances in the field.

This detailed new edition provides a comprehensive collection of protocols applicable to all members of the Coronavirinae sub-family currently and that are also transferrable to other fields of virology.

1. Introduction to in silico Modeling to Study ROS DynamicsJana Schleicher 2. Assessment of ROS Production in Mitochondria of Live CellsPlamena R. Angelova, Albena T. Dinkova-Kostova, and Andrey Y. Abramov 3. Measurement of Superoxide Production in Acute Hypoxia by Fixed-Cell MicroscopyPablo Hernansanz-Agust├¡z, Carmen Choya-Foces, and Antonio Mart├¡nez-Ruiz 4. A Photodynamic Tool to Promote a Sustained, ROS-Dependent Growth of Human Hair Follicles in ex vivo CultureMar├¡a I. Calvo-S├ínchez, Sandra Fern├índez-Martos, and Jes├║s Espada 5. Singlet Oxygen and Protochlorophyllide Detection in Arabidopsis thalianaLiangsheng Wang and Tatjana Kleine 6. Characterization of oxidative lipidomics and autophagy induction in Chlamydomonas reinhardtii under abiotic stressCristina Ortega-Villasante, ├üngel Bar├│n-Sola, Margarita Toledo-Basantes, Flor Mart├¡nez, and Luis E. Hern├índez 7. Fluorescence Detection of Increased Reactive Oxygen Species Levels in Saccharomyces cerevisiae at the Diauxic Shift Abhishek Sinha and Elah Pick 8. Flow Cytometry and Confocal Microscopy for ROS Evaluation in Fish and Human SpermatozoaVanesa Robles, Marta F. Riesco, Juan M. Mart├¡nez-V├ízquez, and David G. Valcarce 9. Measurement of Reactive Oxygen Species In Semen Samples Using ChemiluminescenceT├ónia R. Dias 10. Methods to Unravel Pathways of Reactive Oxygen Species in the Photodynamic Inactivation of BacteriaNatalia S. Gsponer and Edgardo N. Durantini 11. Revealing ROS Production by Antibiotics and Photosensitizers in Biofilms. A Fluorescence Microscopy ApproachSol R. Mart├¡nez  and Andr├⌐s M. Durantini 12. EPR Spectroscopy as a Method for ROS Quantification in SkinSilke B. Lohan, Daniela Ivanov, Nadine Sch├╝le, Bastian Berger, Stephanie Albrecht, and Martina C. Meinke 13. Detection of Superoxide Radical in Adherent Living Cells by Electron Paramagnetic Resonance (EPR) Spectroscopy Using Cyclic Nitrones Kahina Abbas, Nikola Babi─ç, and Fabienne Peyrot 14. Singlet Oxygen Quantum Yield Determination Using Chemical AcceptorsRoger Bresol├¡-Obach, Joaquim Torra, Renzo P. Zanocco, Antonio L. Zanocco, and Santi Nonell 15. A General Method to Establish the Relative Efficiency of Different Sonosensitizers to Generate ROS for SDTViviana Grosso, Alejandro Senz, and Arnaldo T. Soltermann  16. Lipid Peroxidation Assay using BODIPY-phenylbutadiene Probes:A Methodological OverviewJuan C. Stockert  17. Use of Fluorescent Probes to Detect ROS in Photodynamic TherapySulbha K Sharma and Michael R Hamblin 

This updated edition emphasizes the benefits of structural genomics to the wider structural research community with a diverse range of applicable methods.

This volume provides a varied selection of current methods and molecular probes used to generate, image and quantify ROS production and lipid peroxidation. Chapters guide readers through methods and protocols on in silico modelling of ROS dynamics in biological systems and a breaking proposal. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge,  Reactive Oxygen Species: Methods and Protocols aims to ensure successful results in the further study of this vital field.

This updated edition emphasizes the benefits of structural genomics to the wider structural research community with a diverse range of applicable methods.

Part I: Introduction 1. Modified Forms of Cytosine in Eukaryotes: DNA (De)Methylation and Beyond            Maria Eleftheriou and Alexey Ruzov 2. Evidence for Non-Cytosine Epigenetic DNA Modifications in Multicellular Eukaryotes: An Overview            Paige Lowe, Ryszard Olinski, and Alexey Ruzov 3. Mass Spectrometry-Based Analysis of DNA Modifications: Potential Applications in Basic Research and Clinic            Ryszard Olinski, Rafal Rozalski, and Daniel Gackowski 4. Mapping DNA Methylation in Mammals: The State of the Art            Antonio Lentini and Colm E. Nestor Part II: Detection and Quantification of DNA Modifications Using Mass Spectrometry and Capillary Gel Electrophoresis 5. Detection of Modified Bases in Bacteriophage Genomic DNA            Yan-Jiun Lee and Peter R. Weigele 6. Liquid Chromatography-Mass Spectrometry Analysis of Cytosine Modifications            Nan Dai and Ivan R. Corr├¬a Jr. 7. Detection of DNA Methylation in Genomic DNA by UHPLC-MS/MS            Konstantinos Boulias and Eric Lieberman Greer 8. Quantification of DNA Modifications Using Two-Dimensional Ultra-Performance Liquid Chromatography Tandem Mass-Spectrometry (2D-UPLC-MS/MS)            Marta Starczak, Maciej Gawronski, Ryszard Olinski, and Daniel Gackowski 9. MS Analysis of DNA Modifications in Urinary/Body Fluids            Aleksandra Skalska, Agnieszka Siomek-Gorecka, Ryszard Olinski, and Rafal Rozalski 10. Preparation of Internal Standards for 2D-UPLC-MS/MS Quantification of Non-Canonical DNA Bases            Marta Starczak, Aleksandra Skalska, Rafal Rozalski, Ryszard Olinski, and Daniel Gackowski 11. Detection of TET-Oxidized 5-Methylcytosine Bases by Capillary Gel Electrophoresis            Romualdas Vaisvila, Lise Hunault, and Lana Saleh Part III: Assessing the Levels and Spatial Distribution of DNA Modifications Using Antibody-Based Approaches 12. Immunochemical Detection of Modified Cytosine Species in Mammalian Preimplantation Embryos            Tie-Bo Zeng and Piroska E. Szab├│ 13. Immunochemical Detection of Modified Cytosine Species in Lampbrush Chromatin            Garry T. Morgan 14. Detection of Low-Abundance DNA Modifications Using Signal Amplification-Based Immunocytochemistry            Abdulkadir Abakir and Alexey Ruzov 15. Immunohistochemical Detection of Modified Cytosine Bases in Rodent Brain            Marcus D. Wallis and Rebecca C. Trueman 16. Immunohistochemical Detection of 5-Hydroxymethylcytosine and 5-Ca

Part I: Vector design For vaccination1. A Novel Cre Recombinase Mediated In Vivo Minicircle (CRIM) DNA Vaccine Platform for Veterinary ApplicationYanlong Jiang, Guilian Yang, and Chunfeng Wang 2. Vaccination with Messenger RNA - a Promising Alternative to DNA VaccinationIstv├ín Tomb├ícz, Drew Weissman, and Norbert Pardi 3. Impact of a Plasmid DNA-Based Alphavirus Vaccine on Immunization Efficiency Kenneth Lundstrom Part II: DNA Vaccine Adjuvants and Immunostimulatory Response 4. CpG Oligonucleotides as Vaccine AdjuvantsNeslihan Kayraklioglu, Begum Horuluoglu, and Dennis M. Klinman 5. Molecular Adjuvants for DNA Vaccines: Application, Design, Preparation and FormulationAilar Sabbaghi and Amir Ghaemi 6. Assessing Antigen-specific Cellular Immune Responses Upon HIV/SIV Plasmid DNA Vaccination in the Non-human Primate ModelXintao Hu, Barbara K. Felber, and Antonio Valentin Part III          Biotechnological Processes to Obtain DNA Vaccines7. Enhanced Biosynthesis of Plasmid DNA from Escherichia coli Applying Experimental Design Lu├¡s A. Passarinha 8. Primary Purification of Plasmid DNA Using Differential Isopropanol PrecipitationAlexandra Wagner, A. Rita Silva-Santos, Sara Sousa Rosa, Sophie Gierak, Ana M. Azevedo, and Duarte Miguel F. Prazeres 9. Scale-Up of Plasmid DNA Downstream Process Based on Chromatographic Monoliths Urh ─îernigoj and Ale┼í ┼átrancar 10. Purification of Plasmid DNA by Multimodal Chromatography A. Rita Silva-Santos, Sara Sousa Rosa, Duarte Miguel F. Prazeres, and Ana M. Azevedo 11. Minicircle DNA Vaccine Purification and E7 Antigen Expression AssessmentAna M. Almeida, Dalinda Eus├⌐bio, Jo├úo A. Queiroz, Fani Sousa, and ├éngela Sousa Part IV:         DNA Vaccine Delivery 12. Tumor-Specific CD8+ T Cell Responses Induced by DNA VaccinationPablo C├íceres-Morgado and Alvaro Lladser 13. Therapeutic DNA Vaccine Against HPV16-Associated CancerMeihua Yu and Janin Chandra 14. Bulk and Microfluidic Synthesis of Stealth and Cationic-Liposomes for Gene Delivery ApplicationsLucimara Gaziola de la Torre, Amanda da Costa e Silva de Noronha Pessoa, Bruna Gregatti de Carvalho, Thiago Bezerra Taketa, Ismail Es, and Gabriel Perli 15. Conception of Plasmid DNA and Polyethylenimine Delivery Systems with Potential Application in DNA Vaccines FieldDiana Costa, ├éngela Sousa, R├║ben Faria, Ana Raquel Neves, and Jo├úo A. Queiroz 16. Main Features of DNA Based Vectors for Use in Lactic Acid Bacteria and Update ProtocolsNina D. Coelho-Rocha, Fernanda A. L. Barroso, La├¡sa M. Tavares, Ester S. S. dos Santos, Vasco Azevedo, Mariana M. Drumond, and Pamela Mancha-Agresti Part V:           DNA Vaccine Transfer to Clinical Trials17. Ethics of DNA Vaccines Transfer for Clinical Research Ana Cristina Ramalhinho and Miguel Castelo-Branco 18. Preparation of an Academic Clinical TrialPreparation of an academic clinical trialAna Cristina Ramalhinho and Miguel Castelo-Branco

This book provides an overview of methods and experimental protocols that are currently used to analyze the presence and abundance of non-canonical DNA nucleotides in different biological systems. Focusing particularly on the newly discovered and less studied DNA modifications that are enzymatically produced and are likely to play specific roles in various biological processes, the volume explores chromatography- and mass spectrometry-based techniques for the detection and quantification of DNA modifications, antibody-based approaches to study their spatial distribution in different cells and tissues, and methods to analyze their genomic distribution with the help of bioinformatics tools that interrogate the corresponding datasets. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and comprehensive, DNA Modifications: Methods and Protocols serves as an ideal guide to research scientists and PhD students in this rapidly developing discipline, and, thus, will ultimately contribute to deciphering the roles of non-canonical DNA nucleotides in different biological systems.

This volume details practical procedures on the latest DNA vaccine technology. Chapters guide readers through methods and protocols on DNA vaccine design, the adjuvant influence, production and purification methodologies, delivery systems, and approaches of the influence of DNA vaccines in the immunological response performance and in the cancer immunotherapy. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Vaccines: Methods and Protocols aims to ensure successful results in the further study of this vital field.

1. Use of Yeast Plasmids - Transformation and Inheritance AssaysAnastasiia Mereshchuk, Joyce S.K. Chew, and Melanie J. Dobson 2. Construction of Tight Conditional Mutants Using the Improved Auxin-inducible Degron (iAID) Method in the Budding Yeast Saccharomyces cerevisiaeSeiji Tanaka 3. CRISPR Nickase-Mediated Base Editing in YeastKouichi Kuroda and Mitsuyoshi Ueda 4. Genomic Promoter Shuffling by Using Recyclable CassettesXuelei Tian, Wenqing Zhang, and Wei Xiao 5. Study Essential Gene Functions by Plasmid ShufflingLi Fan and Wei Xiao 6. Scarless Genomic Protein Labeling in Saccharomyces cerevisiaeQian Wang, Yu V. Fu, and Wei Xiao 7. High-Copy Yeast Library Construction and High-Copy Rescue Genetic Screen in Saccharomyces cerevisiae Fanli Zeng and David G. Quintana  8. Genome-Wide Imaging-Based Phenomic Screening Using Yeast (Saccharomyces cerevisiae) Strain CollectionsXiuling Cao, Xuejiao Jin, and Beidong Liu 9. Applications of Oxford Nanopore Sequencing in Schizosaccharomyces pombe Ming He, Xu Chi, and Jie Ren 10. Measuring Genome-Wide Nascent Nucleosome Assembly Using ReIN-MapZhiyun Xu, Jianxun Feng, and Qing Li 11. Targeted Integration of Complex Genetic Elements at Multi-Copy Loci by Golden Gate AssemblyYixuan Zhu and Limin Cao 12. Chromosomal Rearrangements of Synthetic Yeast by SCRaMbLEZhouqing Luo, Shuangying Jiang, and Junbiao Dai 13. Use YeastFab to Construct Genetic Parts and Multicomponent Pathways for Metabolic EngineeringShuangying Jiang, Zhouqing Luo, and Junbiao Dai 14. A Versatile Protocol to Generate Translocations in Yeast Genomes using CRISPR/Cas9Nicolas Agier, Aubin Fleiss, St├⌐phane Delmas, and Gilles Fischer15. Yeast Nucleoplasmic Extracts and an Application to Visualize Chromatin Assembly on Single Molecules of DNA Yong Wang and Yu V. Fu 16. Assays for Autophagy III: Observing Dynamic Protein TraffickingJing Zhu and Zhiping Xie 17. Isolation of Aged Yeast Cells Using Biotin-Streptavidin Affinity PurificationXuejiao Jin, Xiuling Cao, and Beidong Liu 18. High-Throughput Rapid Yeast Chronological Lifespan AssayAbd-Al-Wahab A. Khawaja, Zach R. Belak, Christopher H. Eskiw, and Troy A. A. Harkness 19. Long-Term Imaging and Dynamic Analysis of Cytoophidia in YeastShanshan Zhang, Hui Li, and Ji-Long Liu 20. Monitoring 5''-end Resection at Site-specific Double-strand Breaks by Southern Blot AnalysisHaoyang Peng, Simin Zhang, and Xuefeng Chen   

This fourth edition provides all new methods on yeast that have not been included in the past three editions. Divided in four parts, chapters guide readers through general applications for genetic manipulation in yeast cells, genome-wide studies, systematically rearrange the yeast chromosomes, and pathway methods. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.Authoritative and cutting-edge, Yeast Protocol, Fourth Edition aims to compliment previous editions while offering new tools for the yeast research community.

This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology;

This volume presents the latest techniques used to study germ cell tumors (GCTs), including histopathology;

1. Development and Optimization of Clinical Informatics Infrastructure to Support Bioinformatics at an Oncology CenterRanda M. Perkins and Joseph Markowitz 2. Leveraging Pathology Informatics Concepts to Achieve Discrete Lab Data for Clinical Use and Translational ResearchMandy Flannery O''Leary 3. Cohort Identification for Translational Bioinformatics StudiesTiffany A. Lin, Zeynep Eroglu, Rodrigo Carvajal, and Joseph Markowitz 4. Transitioning Clinical Practice Guidelines into the Electronic Health Record through Clinical PathwaysSharjeel M. Hooda and Karen K. Fields 5. Variable Selection for Time-to-Event DataAi Ni and Chi Song 6. Binary Classification for Failure Risk AssessmentAli Foroughi pour, Ian Loveless, Grzegorz Rempala and Maciej Pietrzak 7. Challenges and Opportunities of Genomic Approaches in Therapeutics DevelopmentJaimie S. Gray and Moray J. Campbell 8. Accessible Pipeline for Translational Research using TCGA: Examples of Relating Gene Mechanism to Disease Specific Outcomes Anders E. Berglund, Ryan M Putney, Jordan H. Creed, Garrick Aden-Buie, Travis A. Gerke, and Robert J. Rounbehler  9. Statistical and Bioinformatics Analysis of Data from Bulk and Single-Cell RNA Sequencing Experiments Xiaoqing Yu, Farnoosh Abbas-Aghababazadeh, Y. Ann Chen, and Brooke L. Fridley 10. Investigating Inter- and Intra-Sample Diversity of  Single-Cell RNA Sequencing DatasetsMeghan C. Ferrall-Fairbanks and Philipp M. Altrock 11. Managing a Large-scale Multi-Omics Project: A Team Science Case Study in ProteogenomicsPaul A. Stewart, Eric A. Welsh, Bin Fang, Victoria Izumi, Tania Mesa, Chaomei Zhang, Sean Yoder, Guolin Zhang, Ling Cen, Fredrik Pettersson, Yonghong Zhang, Zhihua Chen, Chia-Ho Cheng, Ram Thapa, Zachary Thompson, Melissa Avedon, Marek Wloch, Michelle Fournier, Katherine M. Fellows, Jewel M. Francis, James J. Saller, Theresa A. Boyle, Y. Ann Chen, Eric B. Haura, Jamie K. Teer, Steven A. Eschrich, and John M. Koomen 12. Synergistic Drug Combination Prediction by Integrating Multi-omics Data in Deep Learning ModelsTianyu Zhang, Liwei Zhang, Philip R.O. Payne, and Fuhai Li 13. Introduction to Multi-Parametric Flow Cytometry and Analysis of High-Dimensional DataJames Sun, Jodi L. Kroeger, and Joseph Markowitz  14. High Dimensional Flow Cytometry Analysis of Regulatory Receptors on Human T cells, NK cells, and NKT Cells*Ryosuke Nakagawa, Jason Brayer, Nicole Restrepo, James J. Mul├⌐, and Adam W Mailloux  15. Quantitative Analysis of Bile Acid with UHPLC-MS/MSYuan Tian, Jingwei Cai, Erik L. Allman, Philip B. Smith, and Andrew D. Patterson 16. Sample Preparation and Data Analysis for NMR-based MetabolomicsTapas K. Mal, Yuan Tian, and Andrew D. Patterson 

This volume introduces Translational Bioinformatics as it relates to therapeutic development, and addresses the techniques needed to effectively translate large data sets to relevant biological networks. Chapters detail clinical informatics infrastructure, and leverage pathology, immunology, pharmacology, genomic, proteomic, and metabolomic informatics approaches.   Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Translational Bioinformatics for Therapeutic Development: Methods and Protocols aims to ensure success in the study of Translational Bioinformatics.

This detailed book explores a diverse range of topics related to wound healing. Some areas include wound generation as a tool to study cellular interactions and growth, mechanisms by which different tissues regenerate, ischemic wounds and their treatment, repairing corneal wound, as well as a number of emerging technologies to help promote wound regeneration, such as techniques with miRNA, nanomaterials, biomaterials, and stem cell therapies. Written for the highly successful Methods in Molecular Biology series, chapters feature introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Wound Regeneration: Methods and Protocols serves as a vital collection of wound model development and regeneration studies that will be of great use in both the academic and industrial fields of research.

1. Investigating Epidermal Interactions through an In Vivo Cutaneous Wound Healing Assay            John L. Zemkewicz, Racheal G. Akwii, Constantinos M. Mikelis, and Colleen L. Do├ºi 2. A Murine Incisional Fetal Wound Healing Model to Study Scarless and Fibrotic Skin Repair            Traci A. Wilgus 3. Development of Cutaneous Wound in Diabetic Immunocompromised Mice and Use of Dental Pulp-Derived Stem Cell Product for Healing            Carl Greene and Hiranmoy Das 4. Musculoskeletal Tissue Engineering Using Fibrous Biomaterials            George Tan, Yingge Zhou, and Dilshan Sooriyaarachchi 5. Cutaneous Wound Generation in Diabetic NOD/SCID Mice and the Use of Nanofiber-Expanded Hematopoietic Stem Cell Therapy            Sarah Anderson and Hiranmoy Das 6. Contusion Rodent Model of Traumatic Brain Injury: Controlled Cortical Impact            Marie-Line Fournier, Tifenn Cl├⌐ment, Justine Aussudre, Nikolaus Plesnila, Andr├⌐ Obenaus, and J├⌐r├┤me Badaut 7. Evaluation of MicroRNA Therapeutic Potential Using the Mouse In Vivo and Human Ex Vivo Wound Models            Xi Li and Ning Xu Land├⌐n 8. Cellular Migration Assay: An In Vitro Technique to Simulate the Wound Repair Mechanism            A.K.M. Nawshad Hossian and George Mattheolabakis 9. In Vivo Ear Sponge Lymphangiogenesis Assay            Racheal G. Akwii, Md S. Sajib, Fatema T. Zahra, Hanumantha R. Madala, Kalkunte S. Srivenugopal, and Constantinos M. Mikelis 10. Identification of Rho-GEF and RhoA Activation by Pull-Down Assays            Md S. Sajib, Fatema T. Zahra, Racheal G. Akwii, and Constantinos M. Mikelis 11. Wound Matrix Stiffness Imposes on Macrophage Activation            Pu Duann and Pei-Hui Lin 12. Generation of Acute Hind Limb Ischemia in NOD/SCID Mice and Treatment with Nanofiber-Expanded CD34+ Hematopoietic Stem Cells            Derek Barthels and Hiranmoy Das 13. Electrospun Aligned Co-Axial Nanofibrous Scaffold for Cardiac Repair            Divya Sridharan, Arunkumar Palaniappan, Britani N. Blackstone, Heather Powell, and Mahmood Khan 14. Generation of Myocardial Ischemic Wounds and Healing with Stem Cells            Daniela Rolph and Hiranmoy Das 15. Corneal Repair Models in Mice: Epithelial/Mechanical Versus Stromal/Chemical Injuries            Peipei Pan and Matilda F. Chan 16. Topical Adoptive Transfer of Plasmacytoid Dendritic Cells for Corneal Wound Healing            Arsia Jamali, Brendan M. Kenyon, Gustavo Ortiz, Betul N. Bayraktutar, Victor G. Sendra, and Pedram Hamrah&nbs

This book merges approaches in understanding the function of the light-gated ion channels known as channelrhodopsin together with methods addressing how channelrhodopsins can be used to address biomedical questions on a cellular or organismal level.

1. Identifying Genotype-Phenotype Correlations via Integrative Mutation Analysis Edward Airey, Stephanie Portelli, Joicymara S. Xavier, YooChan Myung, Michael Silk, Malancha Karmakar, Jo├úo P. L. Velloso, Carlos H. M. Rodrigues, Hardik H. Parate, Hardik H. Parate, Anjali Garg, Raghad Al-Jarf, Lucy Barr, Juliana A. Geraldo, P├ómela M. Rezende, Douglas E.V. Pires, and  David B. Ascher  2. Machine Learning for Biomedical Time Series Classification: From Shapelets to Deep LearningChristian Bock, Michael Moor, Catherine R. Jutzeler, and Karsten Borgwardt 3. Siamese Neural Networks: An OverviewDavide Chicco 4. Computational Methods for Elucidating Gene Expression Regulation in BacteriaKratika Naskulwar, Ruben Chevez-Guardado, and  Lourdes Pe├▒a-Castillo 5. Neuro-evolutive Algorithms Applied for Modeling Some Biochemical Separation ProcessesSilvia Curteanu, Elena-Niculina Dragoi, Alexandra Cristina Blaga, Anca Irina Galaction, and Dan Cascaval 6. Computational Approaches for de novo Drug Design: Past, Present, and FutureXuhan Liu, Adriaan P. IJzerman, and Gerard J. P. van Westen  7. Data Integration Using Advances in Machine Learning in Drug Discovery and Molecular Biology Irene Lena Hudson 8. Building and Interpreting Artificial Neural Network Models for Biological SystemsT. Murlidharan Nair  9. A Novel Computational Approach for Biomarker Detection for Gene Expression based Computer Aided Diagnostic Systems for Breast Cancer Ali Al-Yousef and Sandhya Samarasinghe 10. Applying Machine Learning for Integration of Multi-modal Genomics Data and Imaging Data to Quantify Heterogeneity in Tumour TissuesXiao Tan, Andrew T Su, Hamideh Hajiabadi, Minh Tran, and Quan Nguyen 11. Leverage Large-scale Biological Networks to Decipher the Genetic Basis of Human Diseases Using Machine LearningHao Wang, Jiaxin Yang, and Jianrong Wang 12. Predicting Host Phenotype based on Gut Microbiome using a Convolutional Neural Network ApproachDerek Reiman, Ali M. Farhat, and Yang Dai 13. Predicting Hot-Spots using a Deep Neural Network ApproachAnt├│nio J. Preto, Pedro Matos-Filipe, Jos├⌐ G. Almeida, Joana Mour├úo, and Irina S. Moreira 14. Using Neural Networks for Relation Extraction from Biomedical LiteratureDiana Sousa, Andre Lamurias, and Francisco M. Couto 15. A Hybrid Levenberg-Marquardt Algorithm on a Recursive Neural Network for Scoring Protein ModelsEshel Faraggi, Robert L. Jernigan, and Andrzej Kloczkowski  16. Secure and Scalable Collection of Biomedical Data for Machine Learning ApplicationsCharles Fracchia 17. AI-based Methods and Technologies to Develop Wearable Devices for Prosthetics and Predictions of Degenerative DiseasesMario Malcangi 

This book merges approaches in understanding the function of the light-gated ion channels known as channelrhodopsin together with methods addressing how channelrhodopsins can be used to address biomedical questions on a cellular or organismal level.

This volume presents examples of how Artificial Neural Networks (ANNs) are applied in biological sciences and related areas. Chapters cover a wide variety of topics, including the analysis of intracellular sorting information, prediction of the behavior of bacterial communities, biometric authentication, studies of Tuberculosis, gene signatures in breast cancer classification, the use of mass spectrometry in metabolite identification, visual navigation, and computer diagnosis. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Artificial Neural Networks: Third Edition should be of value to all scientists interested in the hands-on application of ANNs in the biosciences.

1. An Introduction to Patch Clamp RecordingCharlotte L. Hill and Gary J. Stephens 2.Patch Clamp Technology in the 21st Century Jan Dolzer 3. Heterologous Expression of Ion Channels in Mammalian Cell LinesAlistair Mathie, Emma L. Veale, and Robyn G. Holden 4. Electrophysiology on Channel Forming Proteins In Artificial Lipid Bilayers: Next Generation Instrumentation For Multiple Recordings In Parallel Ekaterina Zaitseva, Alison Obergrussberger, Conrad Weichbrodt, Mordjane Boukhet, Frank Bernhard, Christopher Hein, Gerhard Baaken, Niels Fertig and Jan C. Behrends 5. Perforated Whole-Cell Recordings in Automated Patch Clamp ElectrophysiologyKadla R. Rosholm, Kim Boddum, and Anders Lindquist 6. Multi-electrode ArraysRussell Burley and Jenna R M Harvey 7. Utilising Automated Electrophysiological Platforms in Epilepsy Research Carol J. Milligan and Svenja Pachernegg 8. Dynamic Clamp on a Windows PCLorin S. Milescu  9. Combining Whole-cell Patch-clamp Techniques with Single-cell RNA Sequencing Kashif Mahfooz and Tommas J. Ellender  10. Bioluminescence Methodology for Ion Channel StudiesPaul A Wadswort,, Aditya K. Singh, Nghi Nguyen, Clifford Stephan, and Fernanda Laezza 11. Nucleated, Outside-Out, Somatic, Macropatch Recordings in Native NeuronsFrancesco Tamagnini 12. Preparation of Rat Organotypic Hippocampal Slice Cultures Using the Membrane-Interface MethodTimothy W. Church and Matthew G. Gold 13. In Vivo Patch Clamp StudiesYi Zhou, He Li and Zhongju Xiao 14. In Vivo Optogenetics with Stimulus CalibrationLuke T. Coddington and Joshua T. Dudman 15. Single or Double Patch Clamp Recordings in the Ex Vivo Slice Preparation: Functional Connectivity, Synapse Dynamics, and OptogeneticsJean Simonnet, Louis Richevaux, and Desdemona Fricker 16. Optogenetics and Optical Tools in Automated Patch ClampingKim Boddu, Peder Skafte-Pedersen, Jean-Francois Rolland, and Sandra Wilson